四环素和多西环素对人牙周韧带细胞生物学活性的影响

Biological Effects of Tetracycline and Doxycycline on Cultured Human Periodontal Ligament Cells

目的探讨四环素及多西环素对体外培养的人牙周韧带成纤维细胞(HPDLCs)的生物学作用。方法将不同浓度的2种药物(1、5、20、100、500、2500滋g/ml)加入体外培养的HPDLCs中,共孵育2d后,在倒置显微镜下观察其对细胞形态的影响,并用MTT法、考马司亮蓝法及3H-TdR掺入法,分别检测其对细胞的增殖活性、蛋白合成及DNA合成的影响。结果在1～100滋g/ml的浓度范围内,2种药物对细胞形态无影响。在20～100滋g/ml的浓度范围内,四环素显著促进HPDLCs的增殖及生物合成(P﹤0.01),而多西环素只在20滋g/ml时,能显著促进细胞DNA的合成(P﹤0.01)。当2种药物的浓度增至2500滋g/ml时,不仅使细胞的镜下形态发生明显改变,而且严重抑制细胞的生物学活性。结论在合适的浓度范围内,四环素能显著促进HPDLCs的增殖及生物合成,多西环素的促进作用不明显,而浓度过高时两者均具有细胞毒性。

O bjective To explore the biological effects of tetracycline and doxycycline on cu ltured human periodontal ligament cells(HPDLCs).Methods Increasing concentration s of two drugs(1,5,20,100,500,2500ìg/ml)were added to the medium of cult ured HPDLCs separatively.After beening co-incubated for2days,cell morphology was observed un-der reverse microscope;meanwhile,cell proliferation activity was assayed using MTT,the total amount of protein was de-tected by Coumassie Bright Blue method and DNA synthesis was measured by 3 H-TdR.Results Over a concentration range of1to100ìg/ml,cell morphology was normal.Moreover,at a concentration range of20to100ìg/ml,tetracycline significan tly enhanced the proliferative activity and biosynthesis of HPDLCs(P﹤0.01)while doxycycline significantly promoted DNA synthesis only at the concentration of20 ìg/ml.However,higher concentration of the two drugs(2500ìg/ml)not only changed cell morphology under microscope,but also significantly inhibited cellular acti vity.Conclusion The results suggested that proper doses of tetracycline could pr omote proliferation and biosynthesis of HPDLCs,while doxycycline had limited eff ects on biobehavior of HPDLCs.Nevertheless,higher concentrations of the two drug s both had cytotoxic effect.