AccQ-Tag法测定复方精氨酸胶囊中精氨酸的含量

Determination of Arginine in Compound Arginine Capsules Using AccQ-Tag Method

目的:建立测定复方精氨酸胶囊中精氨酸含量的AccQ-Tag法。方法:以6-氨基喹啉-N-羟基琥珀酰亚胺基甲酸酯(AQC)为衍生剂,与复方精氨酸胶囊中精氨酸柱前定量衍生,用Waters HPLC仪,AccQ-Tag^(TM)氨基酸分析柱,以pH4.95醋酸钠缓冲液为流动相A,乙腈-水(3:2)为流动相B,进行梯度洗脱,检测波长为248nm。结果:线性范围:0.1006～0.9054μg,r=0.9995(n=5)。回收率:99.7％,RSD 0.38％(n=5)。结论:本法快速、简便,辅料无干扰,结果满意。

Objective:The paper reports the determination of arginine in compound arginine capsules using HPLC-AccQ Tag method. Methods: The arginine reacted with 6-aminoquinolyl-N-hydroxysuccinimdyl carbaminate (AQC) respectively and quantitatively and produced corresponding derivatives which were analized by HPLC. Chromatographic conditions: AccQ-TagTM for amino acids analysis(3.9×150mm) .mobile phase of program elution(A)sodium acetate solution(pH4. 95),(B)ace-tonitrile: water(3:2)detected at 248nm by UV-detector. Results:The linear response range from 0. 1006~0. 9054ug of arginine (r=0. 9995,n= 5). The mean recoveries of arginine were 99. 7%(RSD:0. 38% ,n = 5) Conclusion: The method is simple, quick and the result of determination is good.