牙周袋厌氧菌检出方法探讨

Study on inspection method of anaerobes in periodontal pockets

作者采用单用非选择性厌氧菌基础培养基（ＣＤＣ）和合用７种选择性厌氧培养基（ＶＳ、ＣＳ、ＢＳ、ＡＳ、ＦＳ、ＥＳ、ＬＳ）两种方法检出牙周袋厌氧菌，均表明Ｇ－厌氧菌是牙周炎的优势菌群。合用选择性培养基较单用ＣＤＣ分离纯化鉴定牙周袋厌氧菌简便、快速、省时，有助于营养苛求或培养困难菌群的分离。实际工作中证明在ＣＤＣ中加入抗生素及各种化学物质制备选择性厌氧培养基，配制方便，适合相应厌氧菌的生长，但建立更多更特异更可靠的选择鉴别培养基仍属必要。

This papaer used two methods only use non-selective medium(CDC)and together with seven selective mediums (VS CS BS AS FS ES LS)to inspect anaerobes in periodontal pockets.The results showed that Gram-negative anaerobes were the predominant microflora of periodontitis.The method of CDC together with selective mediums was founded much simpler and faster,more valuable than the method of only use non-selective medium.It is illustrated that CDC added antibiotics or chemics to make selective mediums was prepared convenience and was suitable for growth of relative anaerobes. But it is still necessary to establish much more, more specical,more reliable selective or identical mediums.