β-榄香烯对人胃癌SGC-7901细胞凋亡及Survivin表达和Caspase酶活性的影响

The Effects of β-elemene on Caspase Activity and Expression of Survivin and Apoptosis of Human Gastric Carcinoma SGC-7901 Cell

目的 观察β 榄香烯对人胃癌细胞系SGC - 790 1凋亡抑制蛋白survivin及caspase酶活性的影响 ,探讨β 榄香烯诱导SGC - 790 1凋亡的作用机制。 方法 用不同浓度 β 榄香烯作用胃癌SGC - 790 1细胞后 ,分别采用Northernblot、Westernblot检测SurvivinmRNA和蛋白水平 ,用酶标仪比色法检测Caspase酶活性 ,分别采用TUNEL法检测细胞凋亡。结果 β 榄香烯能下调SurvivinmRNA和蛋白水平 ,增强Caspase酶活性 ,并诱导胃癌SGC - 790 1细胞凋亡 ,效果呈浓度和作用时间依赖性。结论 β 榄香烯能诱导人胃癌细胞系SGC - 790 1凋亡 ,可能与下调Survivin表达与增强Caspase酶活性有关。

Purpose: To study the expression of Survivn and Caspase activity of human gastric carcinoma cell line SGC - 7901 treated with β-elemene, and to investigate the mechanism of β-elemene to induce tumor cell's apoptosis. Methods: After gastric cancer SGC - 7901 cell were treated with different doses β-elemene, the protein and mRNA level of Survivn were detected by Western blot as well as Northern blot respectively, Caspase activity were evaluated by microplate reader, apoptosis were. studied by TUNEL. Results: β-elemene could down-regulate the level of protein and mRNA of Survivn, and elevate the Caspase activity, and induce gastric cancer SGC - 7901 cell apoptosis. The effects were be relevant to the time and dose of β-elemene. Conclusions: β-elemene could induce SGC - 7901 cells apoptosis by down-regulation of expression of Survivin and up-regulation of Caspase activity.