增生性瘢痕中磷酸化P38丝裂原活化蛋白激酶和c-Jun蛋白的表达特征及其与瘢痕形成的关系

CHARACTERISTICS OF P38 MITOGEN-ACTIVATED PROTEIN KINASE AND c-Jun EXPRESSION IN HYPERTROPHIC SCAR AND THEIR EFFECTS ON SCAR FORMATION

目的 观察磷酸化 P38丝裂原活化蛋白激酶 (P38MAPK)和 c- Jun蛋白在增生性瘢痕组织中的表达特征及其影响瘢痕形成和成熟的机制。 方法 取 16例住院的增生性瘢痕患者的瘢痕组织 ,并将其分为生长活跃期组(形成时间在 1年以内 ,含 1年者 )、生长稳定期组 ,每组各 8例 ;另取正常皮肤 8例。所有取材均未经任何治疗。用免疫组织化学方法和常规病理技术检测磷酸化 P38MAPK和 c- Jun两种蛋白 ,在增生性瘢痕组织和正常皮肤组织中的表达和分布规律。 结果 正常皮肤组织中 ,磷酸化 P38MAPK主要分布于表皮基底层细胞的胞浆和胞核内 ,c- Jun蛋白则主要定位于表皮细胞和内皮细胞中 ,两种蛋白的阳性细胞率分别为 2 1.3%± 3.6 %和 33.4 %± 3.5 %。在处于生长活跃期的增生性瘢痕组织中 ,磷酸化 P38MAPK和 c- Jun的阳性信号主要定位于表皮细胞和部分成纤维细胞内 ,阳性细胞率分别上升至 6 9.5 %± 3.3%和 5 9.6 %± 4 .3% ,明显高于正常皮肤组织 (P<0 .0 1) ;而在成熟稳定的增生性瘢痕中 ,两种蛋白表达有所下降 ,但仍高于正常皮肤组织。 结论 增生性瘢痕的形成和成熟可能与激活 P38MAPK信号传递通路 ,影响c- Jun蛋白表达有关。

Objective To observe the protein expression of phosphorylated form of P38 mitogen activated protein kinase(P38MAPK) and c Jun in hypertrophic scar skin and to explore their influences on the formation and maturation of hypertrophic scar. Methods The expression intensity and distribution of phosphorylated form of P38MAPK and c Jun were examined with immunohistochemistry and pathological methods in 16 cases of hypertrophic scar skin and 8 cases of normal skin. Results In normal skin, the positive signals of phosphorylated form of P38MAPK mostly distributed in basal lamina cells of epidermis, while c Jun was mainly located in epidermal cells and endothelial cells. The positive cellular rates of two proteins were 21.3%±3.6% and 33.4%±3.5% respectively. In proliferative hypertrophic scar skin, the particles of phosphorylated P38MAPK and c Jun were mainly located in epidermal cells and some fibroblasts. The positive cellular rates of two proteins were significantly elevated to 69.5%±3.3% and 59.6%±4.3% respectively ( P< 0.01). In mature hypertrophic scar, the expression of these proteins decreased but was still higher than that of normal skin. Conclusion The formation and maturation of hypertrophic scar might be associated with the alteration of phosphorylated P38MAPK and c Jun protein expression in hypertrophic scar.