摘要
目的 探讨贝那普利防治环孢素A (CsA )慢性肾毒性的机理。方法 给进低盐饮食的SD大鼠灌服CsA ,2 8d后用逆转录聚合酶链反应 (RT PCR)的方法观察肾内转化生长因子 β1(TGF β1)及肾素mRNA表达 ,用免疫组化测定肾组织中TGF β1及四型胶元 (CollagenⅣ )的改变 ,并观察贝那普利对上述指标的影响。结果 使用CsA的大鼠肾内TGF β1及肾素mRNA呈现高表达 ,并伴有CollagenⅣ肾内沉积增加 ;加用贝那普利能抑制TGF β1的表达 ,但对肾素mRNA表达的影响不明显。结论 贝那普利对CsA的慢性肾毒性的防治与其抑制TGF β1mRNA的高表达及减轻肾内CollagenⅣ的沉积有关。
Objective To explore the mechanisms of protective effect of benazepril in the treatment of experimental cyclosporin- induced chronic nephrotoxicity. Methods Rats were on low-salt diet and cyclosporine A (CsA) was administered by gastric gavage at a dose of 30?mg/kg once daily for 28 days. The expression of mRNA for intrarenal transforming growth factor-β1 (TGF-β1) and renin was detected by reverse transcription-polymerase chain reaction (RT-PCR). Intrarenal expression of TGF-β1 and Collagen Ⅳ was determined by immunohistochemistry. The effects of benazepril on these changes were also evaluated. Results Chronic cyclosporine-induced nephropathy may be related to TGF-β1 and renin mRNA up-regulation as well as matrix proteins accumulation in interstitium. Benazepril could reduce TGF-β1 mRNA up- regulation and decrease intrarenal matrix proteins accumulation. Conclusion Decreased CsA-related TGF-β1 up-regulation expression and accumulation of matrix proteins in the kidney may be related to mechanisms of protective effect of benazepril in the treatment of cyclosporin-induced chronic nephrotoxicity.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2003年第5期307-309,共3页
Chinese Journal of Organ Transplantation
基金
上海市医学领先专业重点学科基金资助项目 ( 9830 0 5)
河南省自然科学基金资助项目 ( 0 1110 2 50 0 0 )
