显示小脑普肯耶细胞的染色法

A STUDY OF STAINING TECHNIQUE FOR SHOWING PURKINJE CELLS IN CEREBELLUM

①目的 探讨显示小脑普肯耶细胞形态结构的染色方法。②方法 Golgi镀银法显示普肯耶细胞 ,并与常规的苏木精 伊红染色法相比较。③结果 采用Golgi镀银制片法 ,能够显示出小脑普肯耶细胞的胞体、树突、轴突及树突棘的特殊形态结构 ,普肯耶细胞呈扇形 ,其形态结构十分典型 ,清晰可见。而常规的苏木精 伊红染色只能显示普肯耶细胞的胞体。④结论 Golgi镀银法是显示小脑普肯耶细胞形态结构的理想染色方法。

Objective\ To study a staining method for showing the morphological structure of purkinje cells in cerebellum.\ Methods\ Purkinje cells were stained by Golgi argentaffin technique, the results were compared with the routine staining. Results\ The cell body, large dendritic trees and spine, as well as single axon were shown clearly in a purkinje cell by Golgi argentaffin technique , while by using routine staining, the cell body only be shown.\ Conclusion\ Golgi argentraffin staining is an ideal method in showing morphological structure of purkinje cells of cerebellum.