染色体G显带两种处理方法效果的比较

A COMPARISION OF TWO CHROMOSOME G-BANDING METHODS

①目的 探讨快速稳定显示G显带的方法。②方法 采用染色体制备技术 ,分别用胰蛋白酶磷酸(PBS)法和乙二胺四乙酸胰蛋白酶 (EDTA)法显示G显带。③结果 PBS法与EDTA法显示带纹同样清晰。④结论 PBS法比EDTA法配制试剂简单、预处理条件稳定、结果显示快 ,是一种较好的方法。

Objective\ To explore a rapid and stable method for displaying G banding. \ Methods\ A chromosome preparation technique was adopted, using PBS method and EDTA method to show G banding.\ Results\ Both PBS and EDTA methods could clearly demonstrate the band stria.\ Conclusion\ Compared with EDTA method, PBS method is more simple in preparation of reagent, more stable in preprocessing condition and more rapid in showing the results.