卵巢上皮性癌组织中PTEN mRNA的表达

EXPRESSION OF PTEN GENE IN OVARY EPITHELIAL CARCINOMA

①目的 探讨卵巢上皮性癌组织中PTEN基因的表达及其临床意义。②方法 应用半定量逆转录聚合酶链反应 (RT PCR) ,分别检测 4 9例卵巢上皮性癌组织和 2 0例正常卵巢组织中PTEN基因外显子 5 8和 1 8mRNA的表达 ,同时结合临床病理资料进行分析。③结果 4 9例卵巢上皮性癌组织中PTENmRNA相对表达水平外显子 5 8为 0 .83± 0 .17,外显子 1 8为 0 .78± 0 .14 ;2 0例正常卵巢组织中PTENmRNA相对表达水平外显子5 8为 1.4 2± 0 .2 9,外显子 1 8为 1.37± 0 .2 4 ,两者比较差异有显著性 (t=10 .93、12 .73,P <0 .0 5 )。卵巢上皮性癌组织中PTENmRNA表达水平的降低与卵巢上皮性癌的组织类型、淋巴结有无转移无关 (t =1.32、1.14 ,F =1.14、1.0 4 ,P >0 .0 5 ) ;但随临床分期和组织学分级的增高其表达水平明显降低 (t=2 .5 3～ 2 .99,P <0 .0 5 )。④结论 PTEN基因转录水平的异常在卵巢上皮性癌的发生发展中具有一定的作用。

Objective\ To investigate the expression and its clinical significance of PTEN mRNA in ovary epithelial carcinoma. \ Methods\ The expression of PTEN mRNA was detected by semi quantit ative reverse transcriptase polymerase chain reaction(RT PCR) in 49 cases of ov ary epithelial carcinomas and 20 cases of normal ovarian tissues. \ Results\ For the 49 ovary epithelial carcinomas, the relative mean expression of PTEN mRNA of exon 5 8 and exon 1 8 was 0.83±0.17 and 0.78±0.1 4, respectively. For the 20 normal ovarian tissues, the relative mean expression of exon 5 8 and exon 1 8 was 1.42±0.29 and 1.37± 0.24 , respectively. Sig nificant difference existed between the two groups( t=10.93,12.73;P <0.05). The expression of PTEN mRNA was not correlated with histological type or lymphat ic metastasis ( t=1.32,1.14;F=1.14,1.04; P >0.05), but decreased significan tly with the increase of the clinical staging and histological grading( t=2.53 -2.99, P <0.05). \ Conclusion\ PTEN gene inactivation might play a role in the genesi s and development of ovary epithelial carcinoma.