胃癌和相应癌旁组织中EB病毒感染的检测

DETECTION OF EBV IN GASTR IC CARCINOMAS AND CORRESPONDING PARACARCINOMAS

①目的 探讨Epstein BarrVirus(EBV)感染与胃癌发生发展的关系。 ②方法 应用PCR Southern检测 172例胃癌组织和相应癌旁组织中EBVDNA ,阳性标本进一步用原位杂交技术检测EBV小RNA(EBER1)的表达 ,以确证EBV感染。③结果 172例胃癌组织 11例EBV阳性 ,阳性率为 6 .39% ,EBV阳性率与病人性别、年龄、组织学分型和临床分型之间无明显关系 (P =0 .0 6 9～ 0 .4 85 ) ;172例相应癌旁组织中未检测到EBV基因组的存在 ,胃癌组织及相应癌旁组织EBV阳性率差异有显著意义 (P =0 .0 0 3)。④结论 部分胃癌组织可检测到EBV基因组 ,EBV感染与胃癌的发生有一定的相关性。

Objective\ To study Epstein Barr virus infection in gastric carcinomas and corresponding p aracarcinomas. Methods\ PCR Southern analyses of EBV DNA were applied on 172 gastric carcinomas and corresponding paracarcinomas. The positive specimens were further detected for EBV small RNA (EBER1) in their paraffin embedded sections by in situ hybridization to confirm EBV infection.\ Results\ EVB sequences were detected in 6.39% (11/172) cases of g astric carcinomas but in none of the corresponding paracarcinomas. Statistical a nalysis showed that there was significant difference between them ( P =0.003). There was no significant difference between EBV positive and EBV negative gas tric carcinomas in age, sex, pathological differentiation of clinical stages ( P =0.069-0.485).\ Conclusion\ EBV sequences can be detected in part of gastric carci nomas, which indicates that some gastric carcinomas are associated with EBV infe ction.