摘要
①目的 探讨硒对风湿性心瓣膜病病人谷胱甘肽过氧化物酶 (GPx)基因表达的影响。②方法 风湿性心瓣膜病病人 30例 ,随机分为对照组和实验组 ,各 15例。实验组术前给予亚硒酸钠治疗 2周。对术中缺血前和再灌注后 30min时GPxmRNA的表达阳性率进行测定。③结果 缺血前对照组的阳性表达率为 6 .6 7% ,实验组为 73.33% ,两组比较差异有显著性 (P =0 .0 0 0 2 4 ) ;再灌注 30min后对照组的阳性表达率为 13.33% ,实验组为 93.33% ,两组比较差异有显著性 (P =0 .0 0 0 0 1)。④结论 风湿性心瓣膜病病人补硒可以提高心肌缺血前和再灌注 30min后GPxmRNA的表达水平 ,拮抗自由基对心肌细胞的脂质过氧化损伤作用。
Objective\ To investigate the influence of se lenium on the expression of glutathione peroxidase(GPx) in patients with rheumat ic heart disease.\ Methods\ Thirty patients with rheumatic heart disease were randomi zed to Control Group and Experimental Group, 15 patients being for each group. P atients in Experimental Group received sodium selenite daily, for two weeks. GPx mRNA expression was detected prior to ischemia and 30 minutes after reperfusion during surgery. \ Results\ Both before ischemia and at 30 minutes after myocardial r eperfusion, the Experimental Group had much higher myocardial GPx mRNA expressio n than Control Group ( P =0.000 24, 0.000 01).\ Conclusion\ The supplement of selenium can improve myocardial GPx mRNA expression and defend against damage to myocardium lipid peroxidation caus ed by free radical.
出处
《青岛大学医学院学报》
CAS
2003年第4期439-440,共2页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅科研基金资助项目 ( 1997CA1CBA3 )
