大脑中动脉缺血再灌注后nNOS的表达

EXPRESSION OF nNOS IMMUNOC REATIVE NEURONS AFTER REPERFUSION FOR MIDDLE CEREBRAL ARTERY OCCLUSION

①目的 探讨神经元型一氧化氮合酶 (nNOS)与脑缺血的关系。②方法 制备Wistar大鼠大脑中动脉缺血再灌注模型 ,用苏木精 伊红染色观察缺血后脑组织的形态学改变 ,用免疫组织化学法显示nNOS的变化。③结果 缺血再灌注后皮质及壳尾核nNOS免疫阳性神经元细胞表达增多 ,以再灌注 3h最多 ,6h开始下降。组织学观察可见缺血区内有神经元的变性、肿胀及坏死。④结论 在局灶性脑缺血再灌注早期nNOS表达增多 。

Objective\ To study the relationship between neuronal nitric oxide synthase (nNOS) and cerebral ischemia. Methods\ A Wistar rat model of reperfusion for brain ischemia was made, the morphology of post ischemic brain tissue was investigated by H a nd E staining, the changes of nNOS were revealed by immunohistochemistery. \ Results\ The numbers of nNOS containing neurons in the ipsilateral cortex and caudoputamen increased significantly after reperfusion, which reache d the peak at three hours and started to declined six hours later. Obvious neuro nal swelling, degeneration and necrosis could be seen in ischemic areas.\ Conclusion\ The expression of nNOS increases in the early stage o f focal cerabral ischemia after reperfusion, and it may be involved in neurotoxi c action in acute phase.