大鼠局灶性脑缺血再灌注损伤后β-淀粉样蛋白的表达

EXPRESSION OF β-AMYLOID PROTEIN AFTER REPERFUSION OF FOCAL ISCHEMIA IN RAT BRAIN

①目的 探讨大鼠局灶性脑缺血再灌注损伤后 β 淀粉样蛋白 (β AP)在梗死区的表达。 ②方法 应用免疫组织化学方法观察大鼠大脑中动脉缺血再灌注后存活 6h、1d、2d、3d、7d、14d不同时间 β AP的表达。 ③结果 缺血再灌注后 1d ,β AP在梗死区及其周围表达开始增强 ,7d时达高峰 (F =92 .6 3,q =5 .2 1～ 2 5 .87,P <0 .0 1)。④结论 β AP在脑缺血再灌注损伤区表达增多 ,提示β

Objective To identify the expression of β-amyloid protein (β-AP) in the infarct area after reperfusion of focal ischemia in rat brain. Methods Immunohistochemical technique was used to examine the expression of β-AP at 6 h, 1 d, 2 d, 3 d, 7 d and 14 d after reperfusion of middle cerebral artery occlusion in rat brain. Results Enhanced immunoreactivity was observed in infarct area at 1 d, with a peak at 7 d (F=92.63,q=5.21-25.87,P<0.01). Conclusion The expression of β-AP increases in the infarct area after reperfusion of MCAO, which suggests that β-AP plays an important role in the pathologic changes following reperfusion of ischemic lesions.