PCNA在胃粘膜肠上皮化生、异型增生和胃癌中表达及意义的研究

Expression and significance of proliferating cell nuclear antigen (PCNA) in intestinal metaplasia, dysplasia and gastric carcinoma of gastric mu-cosa

目的 研究增殖细胞核抗原(PCNA)在胃粘膜、肠上皮化生,异型增生及胃癌中的表达。评估胃癌前病变的发展趋势,进一步探讨胃癌发生的分子生物学机制。方法 应用免疫组化技术测定115例患者的PCNA表达情况。其中50例肠上皮化生、15例异型增生、50例胃癌。结果 PCNA的表达从肠上皮化生、异型增生到胃癌呈递增趋势。在肠上皮化生为轻度表达,异型增生以中度表达为主,而胃癌多呈重度和过度表达。PCNA强阳性表达与胃癌分化程度,侵润深度,淋巴结转移密切相关。结论PcNA在胃粘膜不同疾病细胞核内的表达、反映了细胞增殖状态和生长速度、有助于判断肠上皮化生、异型增生的发展趋势及判断与监测胃癌的预后。

Objective To study the expression of proliferating cell nuclear antigen (PCNA) in intestinal metaplasia, dysplasia and carcinoma of gastric mucosa, extimate the tendency of developing precancerous lesions, and evaluate the molecular pathogenesis of the gastric carcinoma occurring. Methods Using the immunohistochemistry we detected the expression of PCNA in 115 patients including 50 patients with intestinal metaplasia, 15 patients with dysplasia and 50 patients with gastric carcinoma. Results The expression of PCNA was found with crescendo tendency from intestinal metaplasia to dysplasia and gastric carcinoma. And intestinal metaplasia showed slight expression, and dysplasia showed middle expression, while gastric carcinoma showed high or over expression. The strong positive expression of PCNA in gastric carcinoma was related with degree of differentiation, depth of infiltration and lymph nodes metastasis. Conclusion The different expression of PCNA in the gastric mucosa diseases reflects the proliferating and growing condition of the cell, and is predication of the developing tendency of intestinal metaplasia, and dysplasia and predicating and observing the prognosis of the patients with gastric carcinoma.