猴血SV40抗体和SV40 DNA携带关系分析

Analysis of the Correspondence between SV40 Antibody and SV40 DNA Fragments in Rhesus Monkeys' Blood

目的 检测 5 7份恒河猴血清及对应的猴血中抗SV4 0抗体、SV4 0DNA的携带情况 ,找出抗体滴度与DNA携带的相关关系。方法 用间接免疫荧光法检测猴血中SV4 0中和抗体 ,聚合酶链反应 (PCR)法检测SV4 0st抗原DNA的携带情况。结果 5 7份猴血清中 ,SV4 0抗体阳性率为 93% (5 3 5 7) ,抗体滴度最高为 1∶12 80 ,最低为 1∶10 ,GMT为 15 9.5 6 ,PCR检测猴血淋巴细胞SV4 0DNA阳性率为 2 4 .5 6 % (14 5 7) ,当抗体为 1 80以下时 ,病毒整合于血细胞 ,1∶80以上时 ,对st抗原基因的整合有抑制作用。结论 恒河猴SV4

Objective To detect SV40 serum antibodies and SV40 DNA fragments in blood samples in the rhesus monkeys for their correlation. Methods SV40 serum antibodies and SV40 DNA fragments in blood samples in the rhesus monkeys were detected by immunofluorescence and polymerase chain reaction respectively. Results The positive rate of SV40 antibody in 57 rhesus monkeys serum samples was 93% (53/57)with a highest titre 1∶1 280 and a lowest titre 1∶10. The GMT was 159.56. The positive rate of SV40 DNA was 24.56%(14/57) in the blood samples. Conclusion There is correlation between the positive rate of SV40 DNA and the positive rate or titres of SV40 antibody in the rhesus monkeys.