γ-射线和秋水仙素对兰州百合(Lilium davidii var.Willmottiae)体细胞诱变的效应

Effects of Gama Rays and colchicine on Mutagenesis in Somaclones of Lilium davidii var. Willmottiae

本文报道了γ-射线和秋水仙素对百合体细胞诱变后的生物学效应和形态变异,并为诱变处理提供较适宜的剂量和浓度。结果指出,诱发幼苗有利变异的有效处理组合是剂量为200—400伦(剂量率100伦╱分)的γ-射线和浓度为2.0mg/l的秋水仙素。试管苗叶色、叶宽,叶长、鳞茎大小、根量和生长势等性状表现变异,在组培条件下这些变异能保持稳定,田间鉴定正在进行中。

Bulb-scales of Lilium davidii var. willmottiae were irradiated with Y-rays (500-2000 R) and their sections were cultured on MS medium supplemented with colchicine ( 1-4mg/l ) , NAA and BA. The regenerated plantlets were also treated with γ-rays (100-500 R) and colchicine (1-4 mg/1). Treatments of scales with colchicine and irradiation of various dosages all showed significant inhibition on the regeneration of plantlets from the cultures. Proliferation of regenerated plantlets which Were treated with colchicine Was also inhibited, especially when colchicine concentration increased to 2.0 mg/1 or more. The proliferation was somewhat enhanced when regenerated plantlets were irradiated with γ-rays of 300 R or less, while it was inhibited in the case of 400 R or more. The LD50 of γ-rays for the plantlets was 400-500 R. The combined inhibiting effect of colchicine and γ-rays on the proliferation of plantlets was greater than their separate impact. Variations in leaf thickness, leaf colour, bulb size, etc. occurred in plantlets treated with colchicine alone or in combination with irradiation. The variations were stable in subculture of the plantlets. The optimal treatment for induction of variations was 200-400 R γ-rays plus 2.0 mg/1 colchicine. The variations kept stable when the plantlets were transplanted into the field, and some of them are being used in breeding programs.