医用脲酶菌的分离与选育

Isolation and Selecting Culture of Urease-Producing Bacteria Used in Therapy

从尿毒症病人肠道中分离出一株脲酶菌,经鉴定为Enterobactergergoviae.纯化菌株经驯化后能以尿素为惟一氮源.经紫外线及硫酸二乙酯诱变处理后,其脲酶活性提高约180%.用非降解性高分子材料聚乙烯醇将该菌包埋在具有半透膜性质的微胶囊内,体外模拟尿毒症病人血清试验表明,250mg湿菌在8h内清除尿素约50mg.

A ureaseproducing strain obtained from the intestines of uraemic patients was identified as the strain of Enterobacter gergoviae. The strain made use of the urea as sole nitrogen source after cultured, and the urease activity of mutant strain treated by multiple mutagenesis(UV,DES) was 180% higher than that of original strain. The strains were entrapped into semipermeable microcapsules which were made of polyvinyl alcohol, an undegraded polymer material. The experimental results obtained from simulating serum with the urease strain entrapped by microcapsule proved that 250 mg wet weight cell could clear away 50 mg urea in 8 hours.