苹果浓缩汁中耐热菌的分离及其生长特性的初步研究

Isolation of Thermotolerant Bacteria from Apple Juice Concentrate and Their Growth Characteristics

采用酸化的凯氏培养基对苹果浓缩汁中的耐热菌进行分离培养,并对它们的生理生长特性进行了初步研究。这些耐热菌并不是一种单一形态的菌,而是表现为多形态的。三株耐热菌菌株ATB-1、ATB-2、ATB-7形态及培养特征虽不尽相同,但均可以在25～50℃温度范围内生长,最适生长温度范围为40～50℃。生长pH值范围分别为3.0～6.0、2.5～6.0、2.5～6.0,而最适生长pH值范围则分别为3.5～4.5、3.0～4.0、3.0～4.0。它们的芽孢均具有较强的抗热性,其D90℃值分别为10.4、19.8、26.5min。

In this paper,the thermotolerant bacteria was isolated from apple juice concentrate and incubated with Kirin-mediumacidified with malic acid.The growth and physiological characteristics of the three thermotolerant bacteria strains ATB-1, ATB-2 and ATB-7 were studied.These thermotolerant bacteria showed as non- single form of bacterium, but a polymorphouscommunity of bacteria.They can grow in a temperature range of 25℃ to 50℃ and pH range of 3.0 to 6.0,and the optimaltemperature and pH range for growth are 40℃ to 50℃and 3.0 to 4.0 respectively. Their spores were fairly heat resistant, andthe D90℃ value of the spores of ATB-1, ATB-2 and ATB-7 is 10.4,19.8,26.5 minutes respectively as estimated.