乌桕叶水溶性多糖蛋白复合体及其组成的研究

Studies on Soluble Glycoprotein and Component of Chinese Tallow Leaf

为了查明乌桕叶水溶性多糖成分,采用溶剂提取分离,Sephadex G-100纯化并测分子量,UV-Visible光谱检测组分,UV、IR测定其连接方式,PC、TLC、IEC测定其组成及比例。结果表明:该水溶性多糖为糖蛋白复合体,平均分子量为53000;多糖与蛋白质组成比为94:6;含有半乳糖、葡萄糖、鼠李糖、阿拉伯糖、木糖,单糖摩尔组成比为25:24:18:31:2;蛋白质部分以甘氨酸、丙氨酸、丝氨酸等中性氨基酸为主。含有O-型糖肽键和α-糖苷键。同时还测定了其抗氧化活性。乌桕叶含有一种以多糖成分为主的中性杂多糖蛋白复合体,且具有较强的清除羟自由基活性。

To investigate soluble polysaccharide of Chinese tallow leaf, a glycoprotein was extracted and isolated from Chinesetallow leaf. The glycoprotein was purified by Sephadex G-100. The average molecular weight of the glycoprotein was 53000.Quality ratio of saccharide and protein was 94:6 using sulphuric acid-phenol method and amino acid analysis. PC and TLC ofthis glycoprotein indicated the saccharide component that consisted of galactose, glucose, rhamnose, arabinose, xylose with themolar ratio of 25:24:18:31:2 respectively. Amino acid analysis of this glycoprotein showed that Gly,Ala,Ser etc. were mainlyneutral amino acid constituent. The characteristics of the glycoprotein by IR analysis showed the typical absorption ofpolysaccharide and indicated the presence of α-glycoside linkage. The β-Elimination reaction demonstrated the existence ofO-glycoside linkage in this glycoprotein. At the same time, the antioxidant activity was also investigated. This was a glycoproteinof hybrid neutral polysaccharide with hydroxyl radical scavenging activity.