拟除虫菊酯类农药降解菌的紫外线诱变

Mutation of synthetic pyrethroid insecticides-degrading strains by ultraviolet radiation

拟除虫菊酯类农药降解菌阴沟肠杆菌 (Enterobactercloacap)w10 j15菌株经紫外线诱变后 ,筛选出突变株 5 5株。分别在 30℃ ,转速 180rpm条件下培养 3d ,测降解力 ,获得正突变菌株 6株 ,经加药普通斜面传种 10代 ,2株 (UW 19,UW 2 )降解力保持稳定。降解力较强的UW 19对联苯菊酯、甲氰菊酯、氯氰菊酯的降解率分别达 70 .4 0 %、84 .0 4 %、70 .87% ,比出发菌株降解率提高了近 2 0 % ;UW 2也比出发菌株提高了约 10 %的降解率。

After mutating w10j15, a strain of Enterobacter cloacap which can degrade pyrethroid insecticides including bifenthrin, fenpropathrin and cypermethrin, by ultraviolet radiation treatment, 55 mutants were obtained. Cultured for 3 days under 30℃ and shaking at the rate of 180 rpm, their degradation capacities were determined. Based on the degradation efficiency, 6 positive mutants were obtained. Two of these positive mutants kept stable degradation capacity after being generated for 10 generations on the tube medium containing synthetic pyrethroid insecticides. One mutant named UW19 had the degration rates of 70.40%, 84.04%, 70.87% to bifenthrin, fenpropathrin, cypermethrin respectively, which were higher 20% than the original strain's. Degradation capacity of other mutant, UW2, had also increased about 10%.