多效唑对草莓种质离体保存的影响

Effect of Paclobutrazol on Conservation of Strawberry Germplasm in Vitro

取离体培养形成的草莓单芽接种到 1/ 2MS附加不同浓度多效唑和 0 5mg/L6 BA的培养基上 ,研究了多效唑对草莓试管苗生长及其保存的影响。结果表明 ,多效唑对草莓试管苗芽的分化有明显的促进作用 ,对草莓试管苗的伸长具明显的抑制作用 ,当多效唑浓度为 0 0 4mg/L ,苗高为对照 (多效唑浓度为 0mg/L)的 2 9%～ 6 3%,当多效唑浓度较高 (0 2mg/L)时 ,延迟了试管苗的发根 ,且抑制根的伸长。在继代培养中 ,多效唑抑制芽的分化 ,同时抑制苗的生长。在本试验中 ,不同浓度的多效唑对草莓试管苗保存成活率差异不十分明显 ,但野生草莓品种在离体保存中多效唑浓度不宜高 ,以 0 1mg/L为好。

In vitro conservation of strawberry germplasm at 25℃ was studied by adding paclobutrazol in 1/2MS+BA 0.5mg/L medium. The results showed that under the presence of paclo butrazol the growth potential of strawberry plantlets in vitro could be decreased, the shoots rate of that could be increased, but the roots rate of that would be reduced. In subculture paclobutrazol could inhibit the bud shooting and the plantlet growth. The survival rates of the plantlets had no obvious differences under the different concentrations of the paclobutrazol in the culture medium, but the low level of paclobutrazol was good for the conservation of wild strawberry germplasm.