小麦EST-SSRs的通用性研究

Study on the Transferability of Wheat EST-SSRs

小麦EST SSRs是一种从小麦ESTs序列中开发的新型SSR标记。根据小麦ESTs设计的 5 97对EST SSRs引物在小麦、玉米、水稻和大豆中的有效扩增比率分别为 80 %、6 5 8%、6 2 1%和 5 8 1%。其中 ,2 5 5对EST SSRs引物 (42 7%)在四种作物中都能获得预期产物。序列相似性比较发现 ,2 5 5个通用EST SSRs标记所对应的基因 80 %功能已知 ,其中 30 %与蛋白质定位相关 ,其余 70 %参与细胞代谢、转录调控、细胞发育、防御体系及其他所涉及的生理生化过程。由于不同物种间基因保守性 ,本研究发掘的EST SSRs引物对于小麦功能基因组和比较基因组研究都有重要意义。

EST-SSRs, a new kind of microsatellites (SSRs), was derived from expressed sequence tags (ESTs). A total of 597 EST-SSRs primer pairs were developed in this study, of which about 80%, 65.8%, 62.1% and 58.1% amplified products successfully in wheat, maize, rice and soybean, respectively. A total of 255 primer sets (42.7%) worked across the four species. Sequence similarity alignment of the 255 universal ESTs containing SSRs against database in GenBank indicated that 63 ESTs (30%) were associated with protein destination, and the rest were involved in metabolism, transcription, cellular development, defense system and other pathways related. It is of significance that these EST-SSRs markers will be highly valuable not only for wheat genomics also for comparative genomics among species.