改性碳纤维及表面含氧官能团对反硝化菌固着化的影响

Effect of surface oxygenic functional groups of modified carbon fibres on immobilization of denitrifying becterium

通过对比各种碳纤维表面含氧官能团对反硝化菌的固着化实验,研究了碳纤维表面含氧官能团对反硝化菌固着化的影响。实验结果表明反硝化菌在PAN基碳纤维上的平均挂膜厚度明显高于沥青基和粘胶基碳纤维,碳纤维上适量的表面官能团有助于较多、较牢固地固着反硝化菌。对3种改性PAN基碳纤维C1s的XPS谱图分析得出表面含氧官能团OCO对反硝化菌的挂膜影响最大。当表面含氧官能团OCO中碳原子占碳纤维表面碳原子总量的3%左右时反硝化菌挂膜较佳。经过比较表明PAN基高强度碳纤维是一种生物相容性较好,可长期循环使用及性能优异的反硝化菌固着化载体。

By immobilization of denitrifying bacterium on different kinds of carbon fibers, effect of surface oxygenic functional groups of modified carbon fiber on denitrifying bacterium was investigated. Study showed that Polyacrylonitrile (PAN)based carbon fiber was better than pitchbased and viscosebased carbon fibers for immobilization of denitrifying microorganism. And while surface oxygenic functional groups of carbon fibers were kept at a proper content, immobilized denitrifying microorganism was more and firmer on the surface of carbon fibers. Xray photoelectron spectrum (XPS) analysis of three kinds of PANbased carbon fibers revealed that effect of oxygenic functional group like OCO was the most important for immobilized biomass, and immobilized biomass reached max when content of carbon atoms in OCO was about 3% of total content of surface carbon atoms. PANbased high strength carbon fiber modified by air in proper temperature was a kind of good immobilized carrier with well biocompatibility and recycle for denitrifying microorganism.