色/质谱选择离子模式分析烟丝中的香味物质

Analysis of Aroma Components in Cut Tobacco by GC/MS with Selective Ion Mode

采用同时蒸馏法进行前处理,并以喹啉为内标,用气相色谱/质谱选择离子模式法对卷烟烟丝中的香味物质进行了分析研究。分析条件为:色谱柱:HP-INNOWAX(30m×0.25mmi.d.×0.25μmd.f.)毛细管柱;载气:氦气,恒压75kPa;进样口温度:250℃;进样量:1.0μL,不分流进样,不分流时间:0.75min;程序升温:初始温度60℃,以2.5℃/min升至180℃,保持10min,再以5℃/min升至250℃,保持3min;传输线温度:220℃;离子源温度:200℃;电离能:70eV;选择离子扫描,质量扫描范围:33～350amu。共分析了34种香味物质,其中30种香味物质的相对标准偏差小于5%,介于5%～8%的有4个,方法的重现性良好。采用该法对4种市售卷烟烟丝中这34种香味物质的含量进行了对比分析。

The tobacco samples were prepared with simultaneous distillation and extraction and quinoline was used as an internal standard (IS). The aroma components in the samples were analyzed by GC/MS with selective ion mode. The analytical conditions were as follows: the column was a capillary column, HPINNOWAX (30m×0.25mm i.d.×0.25μm d.f.); the carrier gas was helium, constant pressure 75kPa; the injection temperature was 250℃, injection mode was splitless, and injection volume was 1.0μL. Temperature program was that the initial temperature was 60℃, rose to 180℃ at 2.5℃/min., kept for 10 minutes, and then increased to 250℃ at 5℃/min., kept for 3、minutes. Temperatures of transfer line and ionization source were 220℃ and 200℃, respectively. Ionization energy was 70eV. Scanning mode was selective ion and the scanning range of ion mass was from 33 to 350 amu. 34 aroma constituents were quantified, of which the RSDs (on the basis of the ratio of peak area of aroma constitute to the same of the IS) of 30 aroma compositions were less than 5% and those of 4 components were from 5% to 8%. That indicated that the repeatability of the method was good. The contents of the 34 aroma constitutes in 4 commercial cigarettes were also analyzed and compared.