吡嗪酰胺介入凝胶体外抗结核活性及安全性评价

Activity of Pyrazinamide Gel Against Mycobacterium Tuberculosis in Vitro and Eva luation of Its Security

目的 :观察吡嗪酰胺凝胶体外抗结核作用和支气管介入的安全性。方法 :手工法、仪器法分别测定吡嗪酰胺单体、吡嗪酰胺凝胶的最小抑菌浓度 (MIC)和最小杀菌浓度 (MBC)及家兔经支气管介入的安全性试验。结果 :吡嗪酰胺凝胶对人型结核分枝杆菌标准株、牛型结核分枝杆菌、草分枝杆菌MIC值分别为1mg/L、1mg/L、10mg/L ,MBC值分别为10mg/L、10mg/L、40mg/L ;吡嗪酰胺凝胶与吡嗪酰胺单体MIC、MBC值无显著性差异 ;动物安全性试验阴性。结论 :吡嗪酰胺凝胶具有与吡嗪酰胺单体相同的抗结核菌药效 ,卡波姆基质不影响吡嗪酰胺的抗菌活性 ;

OBJECTIVE:To observe the activity of Pyrazinamide gel against Mycob ac terium tuberculosis in vitro and its security in bronchial interventional therap y METHODS:The MIC and MBC of Pyrazinamide and Pyrazinamide gel were measured b y handwork method and instrument method and secuity of Pyrazinamide gel was asse ssed by bronchial interventional therapy in rabbits RESULTS:The MICs of pyrazi namide gel to M tuberculosis H37 RV,M bovis and M phlei were 1mg/L,1mg/L,1 0mg/L,the MBCs of Pyrazinamide gel to M tuberculosis H37RV,M bovis and M ph lei were 10mg/L,10mg/L,40mg/L respectively;the MIC and MBC of Pyrazinamide gel and those of Pyrazinamide had no significant differences;the animal security ex periment was negative CONCLUSION:These results suggest that Pyrazinamide gel a nd Pyrazinamide have the same efficacy against M tuberculosis,because carbomer dose not affect the activity of Pyrazinamide against M tuberculosis;Pyrazinami de gel which contains carbomer is safe in bronchial interventional therapy