微板法测定抗药性醋酶检测淡色库蚊抗药性的研究

MICROPLATE TEST OF NON-SPECIFIC ESTERASE OF CULEX PIPIENS PALLENS FOR INSECTICIDE RESISTANCE DETECTION

目的 研究简捷灵敏的蚊虫抗药性早期测报技术。方法 以β-乙酸萘酯为底物,坚固蓝B盐为显色剂,用酯酶微板法测定实验室和现场单个淡色库蚊体内非特异性酯酶活力。结果 实验室5个品系淡色库蚊以抗DDVP品系非特异性酯酶活力水平最高,其次为抗残杀威品系和抗DDVP降解品系,抗氯氰菊酯品系较低,与敏感品系相近;3个现场淡色库蚊种群对DDVP抗性为4.15～9.36倍,对残杀威抗性为1.02～3.81倍,其非特异性酯酶活力水平均较高。结论 酯酶微板法测定非特异性酯酶能够检测蚊虫对有机磷和氨基甲酸酯类杀虫剂的抗性水平,能够早期预测抗性发展趋势,便于制订抗药性克服对策。

Objective To explore the simple and sensitive technic for insecticide resistance detection and early prediction. Methods Microplate test was used to detect non-specific esterase(NSE) of single Culex pipeins pallens of laboratory and field population, β-Naphthyl acetate was used as the substrate and fast blue B salt solution as the colour agent. Results The NSE Ievel was highest in anti-DDVP strain, followed by anti-propoxur strain and anti-DDVP degenerated strain, while the Ievel was similar between anti-cypermethrin and susceptible strain. Three field collected CJT. pipiens pallens populations had 4. 15 - 9. 36 fold higher resistance a-gainst DDVP and 1. 02 - 3. 81 fold higher resistance against propoxur than susceptible mosquito, and their NSE Ievel was also higher than susceptible strain. Conclusion Microplate test for NSE can be used for the discrimination of resistance against OP and carba-mate insecticides. The method is easy and rapid to operate with high precision and objective results. Resistance frequency and mecha-nism can be detected at the same time, so early prediction of resistance and working out resistance management measure is possible.