粗大去细胞神经移植物的化学萃取及组织学研究

Research of Acellular Nerve Allograft by Chemical Extraction in Canine

目的 :发展新的化学处理方法 ,清除犬周围神经中的细胞和髓鞘 ,萃取粗大长段的去细胞神经移植物。方法 :以TritonX 10 0和脱氧胆酸钠溶液化学处理犬坐骨神经 ,萃取神经行组织学及免疫组织化学观察。结果 :去细胞神经弹性好 ,细胞和髓鞘被彻底清除 ,雪旺细胞基底板层保留完好 ,成为空的神经基质管。结论 :该方法可有效清除大型哺乳类粗大神经的细胞及髓鞘 ,该神经移植物保持了网管柱状结构 ,保留了雪旺细胞基底板层及其主要成分—板层素。

Objective:To develop a procedure by which cells and myelin in nerve could be removed while the basal lamina tubes remained intact,and to obtain a thick and long acellular nerve allograft in canine.Methods:Ten sciatic nerves were excised from five canines and treated with a solution of Triton X-100 and a solution of sodium deoxycholate at room temperature.The structure of acellular nerves were observed by morphological studies,immunohistochemical studies and transmission electron microscope.Results: Cells,axons and myelin sheath were removed and Schwann cell basal lamina was preserved after extraction procedure.Transmission electron microscopy showed that the myelin sheaths were removed and empty basal lamina tubes remained in the endoneurium.Conclusions:The study developed an extracted procedure,by which cells,axons and myelin sheaths can be removed from a canine sciatic nerve while the basal lamina tubes remained intact and a thick long acellular nerve allograft is obtained.