壮骨止痛冲剂质量标准的初步研究

Primary Study for Quality Standard of Zhuanggu Zhitong (study bone and relieve pain) Granule

目的 :建立壮骨止痛冲剂的质量标准。方法 :采用薄层色谱法 (TL C)对本制剂中元胡进行鉴别 ,采用系数倍率法对本制剂中何首乌游离总蒽醌进行含量测定。结果 :元胡采用相应的前处理方法和层析条件 ,供试品溶液与对照药材溶液在相应位置上有相同斑点。含量测定在线性范围内 r=0 .9996 ,回收率为 96 .0 %,RSD =3.9%。结论 :TL C法可用于壮骨止痛冲剂中元胡的鉴别 ,系数倍率法可用于壮骨止痛冲剂中何首乌游离总蒽醌的含量测定 ,方法准确、灵敏 ,专属性好。

Objective: To establish a standard for quality control of Zhuanggu Zhitong (study bone and relieve pain) Granule. Methods: Using TLC to identify the presence of Rhizoma Corydalis, and K-ratio method to assay the total content of dissociative anthraquinones contained in Radix Polygoni Multiflori. Results: With corresponding pretreatment and chromatography condition, the sample solution and contrasting solution of Rhizoma Corydalis had the same speckle on the relevant location. Within linear range of the mensurated content curve, correlation coefficient r=0.999 6, average recycle rate is 96.0%, and RSD=3.9%. Conclusion: TLC method can be used in identifying the presence of Rhizoma Corydalis. K-ratio method can be used in mensurating total content of dissociative anthraquinones contained in Radix Polygoni Multiflori. It was found that both methods were precise and sensitive, and with good exclusiveness.