76例重庆地区汉族正常人谷胱甘肽S转移酶P1基因多态性研究

Study on genotype polymorphism of glutathione S-transferase P1 among Han nationality in Chongqing

目的 探讨重庆地区正常人谷胱甘肽S转移酶P1(GSTP1) 5号外显子 10 5位氨基酸基因型频率分布情况。方法 用聚合酶链反应 (PCR)技术和限制性片段长度多态性 (RFLP)技术检测 76例重庆地区汉族正常人GSTP15号外显子 10 5位氨基酸基因型和等位基因分布。结果 重庆地区汉族正常人GSTP15号外显子 10 5位点氨基酸基因型分布以野生型纯合子Ile(异亮氨酸 ) 10 5Ile基因型为主 ,占 93.4 2 % ,而杂合子Ile 10 5Val(颉氨酸 )和突变型纯合子Val 10 5Val基因型均较少 ,仅占3.95 %和 2 .6 3%。GSTP15号外显子 10 5位点氨基酸等位基因分布以Ile 10 5等位基因为主 (95 .39% ) ,而Val 10 5等位基因极少 (4 .6 % )。结论 中国重庆地区汉族正常人GSTP15号外显子 10 5位点氨基酸基因型频率和基因频率分布与国外其它民族分布情况有显著性差异 ,与国内其它地区的报道结果也有差异 ,提示在中国汉族人群内部其不同的地区GSTP1基因多态性可能不一致。

Objective To study genotype frequency distribution of glutathione S transferase P1 (GST P1) among Han nationality in Chongqing.Methods GST P1 genotyping was detected by using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 76 normal persons. Results Our study showed that in the genotype frequency of GST P1 homozygous wild type Ile (Isoleucine) 105 Ile was mostly predominant, while heterozygote Ile 105 Val(Valine) and homozygosity mutation Val 105 Val counted little .They were 93.42%, 3.95%, 2.63%,respectively .In allele Ile 105 was predominant, but Val 105 allele was a few.Conclusion There is significantly difference between our study and oversea scholar′s study. It also exists difference between our study and other internal study . It shows there is difference on genotype polymorphism of GST P1 exon 5 105 position amino acid within Chinese population.