胃粘膜线粒体DNA核内整合与bcl-2和bax表达的关系

Mitochondrial DNA integration in the nuclei and expression of bcl-2 and bax mRNA in gastric cancer and its precancerous lesions

目的 探讨胃癌及其癌前病变组织线粒体DNA核内整合与bcl 2和bax基因mRNA表达的关系。方法 采用原位杂交方法检测线粒体DNA核内整合 ;RT PCR方法检测胃癌及其癌前病变组织bcl 2和bax基因mRNA的表达。结果 胃粘膜肠化 (5 3.3% )和异型增生 (70 % )组bcl 2mRNA表达阳性率显著高于浅表性胃炎组 (10 % ) (P <0 .0 5 ) ,而慢性萎缩性胃炎 (5 0 % )和胃癌 (30 % )组bcl 2mRNA表达阳性率与浅表性胃炎组相比则无显著差异 (P >0 .0 5 ) ;胃粘膜异型增生组bcl 2mRNA表达阳性率显著高于胃癌组 (P <0 .0 5 )。异型增生组baxmRNA表达阳性率 (6 0 % )显著高于浅表性胃炎组 (P <0 .0 5 ) ,而萎缩性胃炎(5 0 % )、肠化 (4 6 .7% )和胃癌 (33.3% )组baxmRNA表达阳性率与浅表性胃炎组 (10 % )相比无显著差异 (P >0 .0 5 )。Hp感染组胃粘膜bcl 2和baxmRNA表达阳性显著高于非Hp感染者 (P <0 .0 5 ) ;bcl 2和baxmRNA表达与HpCagA基因表达没有明显的相关关系 (P >0 .0 5 )。mtMSI(+)组bcl 2和baxmRNA表达阳性率与mtMSI(- )组相比无显著差异 (P >0 .0 5 )。结论 线粒体DNA核内整合可能在部分胃癌的发生中起重要作用 ;线粒体DNA核内整合与bcl

Objective To explore the relationship between mitochondrial DNA integration in the nuclei and expression of bcl 2 and bax mRNA in gastric cancer and its precancerous lesions. Methods The sequences of mtDNA in the nuclei were detected by in situ hybridization. expressions of bcl 2 and bax mRNA were detected by RT PCR. Results Expressions of bcl 2 mRNA in intestinal metaplasia(IM,53.3%) and dysplasia (Dys, 70%) were significantly higher than that in chronic superficial gastritis(CSG,10%, P <0.05),no significant differences existed between chronic atrophic gastritis(CAG, 50%) or gastric cancer(GC 30%) and CSG ( P >0.05); expression of bcl 2 mRNA in Dys was higher than that in GC( P <0.05). Significant increased expression of bax mRNA was found in Dys(60%) ( P <0.05) ,but not in CAG(50%),IM(46.7%) and GC(33.3%)( P >0.05),compared with that in CSG (10%). Expression of bcl 2 and bax mRNA in H.pylori infected gastric mucosa was significant higher than that in non H.pylori infected gastric mucosa( P <0.05),whereas expression of bcl 2 and bax mRNA were not consistent with H.pylori CagA status( P >0.05). No significant different expressions of bcl 2 and bax mRNA were found between mtMSI(+) and mtMSI(-)groups ( P >0.05). Conclusions H.pylori infection was associated with increased expression of bcl 2 and bax mRNA in gastric mucosa. mtMSI is independent on expressions of bcl 2 and bax mRNA.