母血中胚胎细胞特异性血红蛋白基因表达

Expression of fetal hemoglobin gene in maternal peripheral blood

目的 检测孕妇外周血中胎儿血红蛋白基因表达 ,为无创伤性产前诊断奠定基础。方法 提取孕妇全血mRNA ,经体外逆转录后 ,用RT -PCR方法 ,应用ε/γ引物引导扩增胎儿血红蛋白基因片段 ,经琼脂糖凝胶电泳后 ,在凝胶成像系统下观察。结果 在 5 0例妊娠妇女中 ,4 4例扩增出ε/γ基因片段 ,6例为阴性。结论 应用RT -PCR方法可直接从孕妇全血中扩增出胎儿血红蛋白基因片段 ,孕妇全血中微量的胎儿mRNA已基本满足体外逆转录及扩增的要求。利用孕妇外周血进行的无创伤性产前诊断具有可行性。

Objective To explore the method for fetal hemoglobin gene expression from peripheral blood of pregnant women.Methods mRNA extracted from peripheral blood of pregnant women was reversed transcript.ε and γ gene was used as a primer to amplify fetal DNA.PCR reactant were analyzed on agarose gels and observed under gel works system.Results 44 samples were amplified with fetal ε and γ gene and 6 with negative in 50 samples.Conclusion RT-PCR methods can be used to amplify ε and γ gene directly from peripheral blood of pregnant women.Little amount of fetal mRNA from maternal blood can be nearly enough for reverse transcription and amplication.Non invasive diagnosis for fetal diseases may be possibility.