人类胚胎冷冻复苏影响因素分析

Analysis of influencing factors on the result of cryopreservation of human embryos

目的:观察患者年龄、促排卵方案、受精方式、胚龄及冻胚移植周期方案对于冷冻胚胎复苏和移植后临床妊娠的影响。方法:慢速冷冻体外受精周期中剩余的质量良好的胚胎,随后于不同周期用快速融解法复苏后进行移植。根据上述因素将患者分组,对复苏后胚胎质量和临床妊娠率进行比较。结果:126例患者中,融解胚胎总数445个,复苏后成活368个(82.7%)。获得临床妊娠48例(38.1%)。年龄对冷冻胚胎复苏率和移植后临床妊娠率的影响比较明显。随着年龄增加,冻胚移植的妊娠率下降,尤其是在35岁以后,下降更为明显。促排卵方案、受精方式、冷冻时胚胎的培养天数和移植周期方案均对冷冻胚胎复苏率和移植后临床妊娠率没有明显影响。结论:在诸多因素中,只有患者年龄会影响冻胚移植的结果。

Objective:To study the effects of age,ovarian stimulation protocol,fertilization method,culture time of the embryos and the transfer cycle on the result of human embryo cryopreservation.Meth-ods:The suitable embryos from in vitro fertilization treatment cycles were cooled down slowly.Then in several kinds of cycles,the embryos were thawed rapidly.According to the above factors,the patients were divided into several groups,and the results were compared.Results:Among the445thawed embryos from126patients,368(82.7%)survived and48(38.1%)patients achieved pregnancy.The age had obvious effect on the result of the cryopreservation of embryos,the pregnant rate declined markedly especially in patients more than35years old.Ovarian stimulation protocol,fertilization method,age of the embryos,and the methods to prepare the endometrium in the transfer cycles had no influence on the result after cryopreser-vation.Conclusion:Among the several factors,only age has influence on the result of the cryopreservation of human embryos.