胃癌端粒酶活性及ras基因突变的研究

The telomerase activity and mutation of ras gene in human gastric carcinoma

目的 检测胃癌及癌前病变组织、胃炎组织端粒酶活性及ras基因变异,了解胃癌发生的生物学特性。方法 应用TRAP法对32例胃癌、16例异形增生、13例肠上皮化生、8例萎缩性胃炎、12例浅表性胃炎进行端粒酶活性及ras基因变异的测定。结果 胃癌组织28例(87.5％)端粒酶表达阳性,ras基因变异27例(84.4％)阳性;异形增生阳性分别为4例(25.0％)和5例(31.3％);肠上皮化生阳性分别为4例(30.8％)和4例(30.8％);萎缩性胃炎阳性分别为1例(8.4％)和2例(16.7％);胃炎组织阳性分别为0和1例(8.3％)。端粒酶及ras基因阳性表达与胃癌的部位、分化度、是否有淋巴转移无关。端粒酶活化与ras基因密切相关。结论 端粒酶活化及ras基因变异均与胃癌发生有关,可为胃癌的诊断提供可靠的指标。

Objective To evaluate the role of telomerase activity and mutation of ras gene in human gastric carcinoma.Method Using PCR-based telomeric repeat amplification protocol telomerase activity and mutation of ras gene was examined in 32 specimens of gastric carcinoma and 16 specimens of heterogenic hyperplasia, 13 specimens of intestinal metaplasia , 8 specimens of atrophic gastritis. Results It was found that the telomerase activity was positive in 28 (87.5 % ) and ras gene was positive in 27 of the 32 human gastric carcinoma specimens (84.4%) .The telomerase activity was positive in 4(25.0%) and ras gene was positive in 5 of the 16 (31.3%) specimens of heterogenic hyperplasia, telomerase activity was positive in 4(30.8%) and ras gene was also positive in 4 of the 13 specimens (30.8%) of intestinal metaplasia, telomerase activity was positive in 1(8.4%) and ras gene was positive in 2 of the 8 specimens (16.7% ) of atrophic gastritis , and telomerase activity was positive in 0 and ras gene was positive in 1 of the 12 specimens (8.3%) of superficial gastritis.The telomerase activity correlated with mutation of ras gene ( P < 0.05) . Conclusion The telomerase activity and mutation of ras gene may play an important role in development and progression of gastric carcinoma and might be a diagnostic marker of human gastric carcinoma.