人巨细胞病毒包涵体——病毒核酸和特异阶段表达抗原的产物

Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus

目的 探讨人巨细胞病毒包涵体的组成及意义。方法 采用显微切割技术结合PCR和Southern杂交及免疫组织化学的催化信号扩增法 ,检测人巨细胞病毒的核酸和 3种不同阶段表达的抗原。结果 通过液压显微切割系统将组织中巨细胞病毒包涵体游离出来 ,PCR扩增出预期长度的DNA片段 ,Southern杂交证实为HCMV的核酸片段 ,催化信号扩增法检测结果显示包涵体主要表达立即早期和早期抗原DDG9/CCH2 ,而基质蛋白AAC10则为阴性。

Objective To study the composition and significance of the inclusion bodies of human cytomegalovirus(HCMV). Methods Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV. Results The inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies. Conclusion The ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.