Iloprost在实验性颅脑损伤中的保护作用

Protective effects of Doprost on the experimental craniocerebral injury

目的:研究Iloprost对大鼠创伤性脑损伤后神经记忆行为及突触体内[Ca2+]液度与脑含水量的影响。方法: 液压冲击致伤装置制作大鼠脑创伤模型,爬杆回避箱测定动物的神经记忆行为,干湿重法测定脑组织含水量,Fura-2/AM荧光标记法测定突触体内[Ca2+]i,并采用Iloprost进行治疗,研究其对神经记忆行为、[Ca2+]i及脑水肿的影响。结果:创伤后动物会发生神经记忆行为的改变、脑组织水含量的增加及钙超载,Iloprost治疗后能改善动物的神经记忆行为,使[Ca2+]i明显下降,脑水肿明显减轻。结论:细胞内钙通道开放、钙离子超载在创伤性脑水肿的发生发展中起了重要作用,Iloprost对创伤性脑水肿有治疗和脑保护作用。

Objective: To explore the effect of Iloprost on neurobehavior, [Ca2+ ] i concentration and brain edema after traumatic brain injury in rats. Methods: The fluid percussion injury instrument was used to establish the brain injury model of rats. We measured the changes of neurobehaviour, [Ca2+ ] i content and water content in brain tissue before and after the administration of Iloprost. Results: After traumatic injury, the neurobehaviour changed, water content increased and [Ca2+ ] i overloaded. After the administration of Iloprost, the neurobehaviour improved, [Ca2+] i content decreased significantly and brain edema improved. Conclusion: It is suggested that [Ca2+ ] i may participate in the pathogenesis of brain edema. Iloprost has protective and care effect on traumatic brain edema by inhibiting the excess of [Ca2+ ] i influx, and decreasing [Ca2* ] i content.