健脑宁对脑中动脉造型大鼠血液流变学指标的影响及机理研究

Studies of Jian Nao Ning (JNN) on Implemented Blood Rheologic Observation and Therapeutically Mechanism in Middle Cerebral Artery Thrombosis Occlusion (MCAO) of Rat

将 6月龄大鼠在麻醉状态下 ,分离出颈内和颈外动脉 ,由颈外动脉向颈内动脉插入直径为 0 3mm的鱼线 15mm ,在血液正常流动状态下保留 12 0min。于造型 2 4h后取出血测定不同剪切速率下的全血粘度、红细胞聚集指数、聚集面积、变形指数、变形面积及Txb2 、6 keto PGF1α、白细胞介素的变化。结果表明 ,大鼠造型 2 4h后血粘度 ,红细胞聚集指数、血栓素升高、IL 1、IL 2、6 keto PGF1α降低 ,Txb2 6 keto PGF1α比值倒置。造型大鼠灌服健脑宁、复方丹参片后上述指标得到明显改善 ,为健脑宁能治疗脑血栓指供了药理学基础。

By using model of MCAO which is made by additional minute line (Φ 3mm) in arteria carotis intema for 120min of anesthetic rat. The experimental result showed that JNN could significantly alleviaete these changes:JNN 11.25g medicinal herbs/kg and 5.625g medicinal herbs/kg could improve the decrease of whole blood viscosity and plasma viscosity (P<0.01, P<0.05 compared with vehicle group); could inhibit of the erythrocyte aggregation (P<0.01, P<0.05 compared with vehicle group); could increase of the erythrocyte deformational activity (P<0.05, compared with vehicle group); could increase content of 6-keto-PGF-{2α} (JNN 11.25g 5.625 medicinal herbs/kg P<0.01, compared with vehicle group) and IL-1 β(JNN 11.25g medicinal herbs/kg P<0.05 compared with vehicle group), IL-2(JNN 11.25g, 5.625 medicinal herbs/kg P<0.05 compared with vehicle group) in blood serum (JNL 11.25g medicinal herbs/kg P<0.05,P<0.05 compared with vehicle group); Could increase of TXBS(JNN 11.25g, 5.625 medicinal herbs/kg P<0.01, compared with vehicle group) in blood plasma.