纳洛酮对体外培养的缺氧大鼠皮质神经元细胞凋亡的影响

Effects of naloxone on rat cortical neuron apoptosis induced by anoxia

目的 :观察缺氧对大鼠皮质神经元细胞凋亡的影响及纳洛酮的保护作用。方法 :取体外培养 12 d的Wistar大鼠皮质神经元细胞 ,随机分为正常对照组、缺氧组、缺氧加纳洛酮组。缺氧 6h后在常氧下继续培养2 4h,用原位末端标记 (TUNEL)法和流式细胞仪检测不同时间段神经元细胞凋亡率。结果 :缺氧能诱导神经元细胞凋亡显著增加 ,纳洛酮可以降低凋亡率 ,与缺氧组相比差异显著 (P<0 .0 1)。结论 :纳洛酮可抑制缺氧诱导的大鼠皮质神经元细胞凋亡 。

Objective: To investigate the damage of anoxia on the cultured rat's c ortical neurons and the protective effects of naloxone. Methods: Cortical neurons cultured for 12 days were randomly divided into three groups: control, anoxic group and anoxic group plus naloxone treated. Cortical neurons were exposed to anox ic environment for 6 hours and then cultured for 24 hours under normoxic condition. The apoptosis o f neurons was detected with the method of TdTmediated dUTP nick end labeling(TUNEL) and the apoptotic rate of neurons was measured using flow cytometry. Results: Anoxia could lead to increasing apoptosis an d death of rate cortical neurons. Naloxone could reduce neuron apoptosis. There was a significant difference between the anoxic group and anoxic group plus naloxone(P<0 01). Conclusion: Naloxo ne can ameliorate the toxicity damage of neurons caused by anoxia through re ducing neuron apoptosis, which suggests that naloxone could protect rat's cortic al neurons from damage under anoxia condition.