FK506滴眼液抑制兔同种异体角膜缘移植排斥反应的免疫病理学研究

Imunopathologic studies on the effects of FK506 eye drops in rabbits of limbal allograft transplantation

目的 :观察同种异体角膜缘移植术后排斥反应的免疫病理学变化 ,探讨FK5 0 6滴眼液的免疫抑制作用。方法 :新西兰兔 48只 48眼建立角膜缘缺陷症动物模型。随机分为PK 5 0 6组、环孢霉素A组 (CsA )及生理盐水对照组 ,每组16眼。 1月后实施同种异体角膜缘移植术。应用免疫病理学方法检测术后 4、 8、 10周角膜缘植片CD2 5的表达和淋巴细胞浸润。结果 :术后 4、 8、 10周三组角膜缘植片CD2 5的表达和淋巴细胞浸润均有升高 ,对照组较FK5 0 6组和CsA组高 (P <0 0 5 ) ,CsA组显著高于FK5 0 6组 (P <0 0 5 )。结论 :PK 5 0 6滴眼液可抑制同种异体角膜缘移植术后植片CD2 5的表达 ,其免疫抑制作用优于CsA。

Objective:To discuss the effects of the immunosuppression of FK506 eye drops on limbal allograft transplantation by observing the immunopathologic process of rejection. Methods:Limbal deficiency models were established in 48 New Zealand rabbits(48 eyes),which were randomized into 3 groups:FK506 group,CsA group and physiological saline control group,each group consisting of 16 eyes.Limbal allograft transplantation was performed one month later.By using immunopathologic techniques,the expression of CD25 and lymphocyte infiltration in grafts were studied the fouth,eighth,tenth week after operation. Results:The expression of CD25 and lmyphocyte infiltration in grafts was increased in all 3 groups.It was higher in control group than the other two groups,and that in CsA group was higher compared with that in FK506 group (P<0 05). Conclusions:Topically administered FK506 eye drops early limbal allograft transplantation can suppress the expression of CD25 in grafts.Its immunosuppressive effects are stronger than CsA.