三七总皂甙对大鼠心肌细胞L-型钙电流的抑制作用

Effect of Panax Notoginseng Saponins on L-tape Calcium Current in Cardiac Myocyte of Rat

目的 :探讨三七总皂甙对大鼠心肌细胞L -型钙电流 (ICa-L)的抑制作用 .方法 :采用全细胞膜片钳方法分别观测不同浓度三七总皂甙对大鼠心肌细胞L -型钙电流的抑制作用 .结果 :PNS 10 0mg/L和30 0mg/L对ICa -L的抑制率分别为 (2 6 73± 4 5 ) %和 (4 9± 19 6 ) % (P <0 0 1) .结论 :三七总皂甙能阻滞大鼠心肌细胞ICa -L。

Obsjective: To study the effect of PNS on L-type Ca 2+ current in isolated cardiac myocytes from rats. Methods:By using whole cell patch clamp recording technique we measured I Ca-L in isolated cardiac myocytes which were streated PNS. Results: The decreasing rate of I Ca-L was(26 7±4 5)% (P<0 01) in 100?mg/L PNS, and (49±19 6)% (P<0 01) in 300?mg/L. Conclusion:PNS can decrease L-type Ca 2+ current of the cardiac myocytes.