Survivin在人原发性肝细胞癌中的表达及其与凋亡的相关性

Expression of survivin gene in human primary hepatic carcinoma and its relotionship with apoptosis

利用免疫组化 (IHC) EnVision法和Tunel法检测了 6 8例原发性肝细胞癌 (HCC)和癌旁肝组织及 1 2例正常肝组织中Survivin、PcNA、Bcl xl,探讨Survivin基因在肝组织细胞中的表达与凋亡指数 (AI)、增殖指数 (PI)和凋亡抑制因子的相关性。结果发现Survivin和Bcl xl在HCC中阳性检测率分别为 5 8.8% (4 0 / 6 8)和 4 8.5 % (33/ 6 8) ,高于癌旁肝组织 [分别为 5 .88%(4 / 6 8)和 36 .8% (2 5 / 6 8) ]及正常肝组织 [0 (0 / 1 2 )和 2 5 % (3/ 1 2 ) ]。Survivin和Bcl xl主要表达在细胞质或细胞膜上。Survivin的阳性表达与HCC的侵袭转移倾向、AI以及Bcl xl有关 (P <0 .0 5 ,P <0 .0 1 ) ,而与PI无关。

The expressions of survivin,PcNA and Bcl xl were detected in 68 patients with pramary hepatic careinoma(HCC) and adjacent tissues and 12 cases of normal hepatic tissues by immunohistochemical (IHC) EnVision and Tunel method.The relationship between survivin gene expression and apoptosis index(AI),proliferation index(PI) and apoptosis inhibition factors were studied.The positive rate of survivin and Bcl xl expression were 58.8%(40/86) and 48.5%(33/68) in HCC,5.88%(4/68) and 36.8%(25/68) in adjacent tissues,0 (0/12) and 25%(3/12) in normal hepatic tissues.The expression of Survivin and Bcl xl are mainly in cell membrane and cytoplasm.Survivin expression is related to metastasis of HCC ( P < 0.05), AI and Bcl xl expressron( P <0.01),but is not related with PI.