一阶导数光谱法测定酚酞片中酚酞的含量

Determination of the Content of Phenolphthalein in Phenolphthalein Tablets with First Order Derivative Spectrophotometry

目的:建立测定酚酞片中酚酞含量的方法,消除杂质荧光母素及辅料的干扰。方法:采用一阶导数光谱法,以225 nm波峰与240 nm波谷振幅值为定量依据。结果:酚酞在0.88-10.56 mg·L-1浓度范围内线性关系良好(r=0.9999),含荧光母素0.10％及1.00％的平均回收率分别为100.12％和100.54％,RSD分别为0.56％和0.74％。结论:该方法可靠,简便快速,结果准确,可消除含量<1.0％的荧光母素的影响。

Objective:To set up a method for the detection of the content of phenolphthalein in phenolphthalein tablets and elimination of the foreign matter fluorane and excipients. Methods: First order derivative spectrophotometry was used in the determination. The quantitative calculations were based on amplitude values of the peak height at 225 run and valley height at 240 nm. Results: A good linear relationship was demonstrated when the concentration of phenolphthalein was in a range of 0.88 - 10.56 mg·L-1 . The correlation coefficient was 0.999 9. The average rates of recovery were 100.12% and 100.54% , with RSD of 0.56% and 0.74% , respectively, when the content of fluorane were 0.10% and 1.00% , respectively. Conclusion :The method was shown to be simple, convenient, rapid, accurate and reliable and capable of eliminating the interference of fluorane with a content below 1.0%.