大肠重复癌微卫星不稳定性研究

Microsatellite instability in multiple primary colorectal cancers

目的 探讨微卫星不稳定性 (MSI)在大肠重复癌与单发大肠癌中的变化规律。方法 PCR SSLP法对 38例大肠重复癌患者的 5 1例癌灶及对照 35例单发大肠癌分别进行 5个碱基序列位点MSI检测。结果 大肠重复癌中复制错误 (RER)阳性率为 5 2 .9% (2 7/5 1) ,对照组为 17.1% (6 /35 ) ,两组差异有非常显著意义 (χ2 =11.2 5 ,P <0 .0 1)。大肠重复癌组中 ,RER阳性与低分化、近端大肠密切关联。RER阳性组与阴性组在年龄、性别、是否伴有转移、Dukes分期上未见异常。结论 MSI在大肠重复癌的发生上起着重要作用 ,MSI可作为预测大肠重复癌发生的有用标志 ,对MSI大肠癌患者应警惕多重癌发生的可能性。

Objective To study the changeable patterns of microsallite instability(MSI)in multiple primary colorectal carcinomas and solitary colorectal tumors.Methods MSI at five microsatellite loci were examined by PCR-SSLP method in 51 tumors from 38 patients with multiple primary colorectal cancers and 35 solitary colorectal tumors. Results The replication errors(RER)+ phenotype was observed in 27 of 51( 52.9% ) tumors from individuals with multiple primary colorectal cancers but only in 6 of 35( 17.1% ) tumors from individuals with a single colorectal cancer. RER positive related tightly with poor differentiation, the proclivity for proximal colon. Conclusion MSI may play an important role in the development of multiple primary colorectal cancers, and may be a diagnostic marker of multiple primary colorectal cancers.