胃癌中p21^(WAF1/CIP1)、细胞周期素D_1、p53表达的相关性

The relationship within the expression of p21^(WAF1/CIP1) 、Cyclin D_1、p53 in gastric carcinoma

目的 探讨p2 1WAF1/CIP1、细胞周期素D1(cyclinD1)、p5 3在胃癌中表达之间的相关性。 方法 应用原位杂交技术检测p2 1WAF1/CIP1mRNA、细胞周期素D1mRNA及免疫组化技术检测p5 3蛋白在胃癌中的表达。结果 p2 1WAF1/CIP1mRNA在癌组织及癌旁正常粘膜中阳性表达率各为 93.15 % (6 8/73)及76 .71% (5 6 /73) ,二者相比具有显著差异 (P <0 .0 5 )。CyclinD1mRNA在癌组织及癌旁正常粘膜中阳性表达率各为 5 4 .79% (40 /73)及 30 .16 % (2 2 /73) ,二者具有显著差异 (P <0 .0 5 )。p5 3蛋白在胃癌中的阳性表达率为 32 .87% (2 4 /73) ,p5 3过表达者 ,其 p2 1WAF1/CIP1mRNA表达较p5 3阴性者为低 ,二者存在显著差异 (P <0 .0 5 )。p2 1WAF1/CIP1表达与细胞周期素D1表达呈负相关。结论 p2 1WAF1/CIP1、CyclinD1、p5 3的异常表达及它们之间可能存在的相互作用 ,对于胃癌的发生发展具有重要意义。

Objective To investigate the relationship within the expression of p21 WAF1/CIP1 、Cyclin D_1 and p53. Methods Hybridyzation in situ and immunohistochemical techniques were used. Results The positive rate of p21 WAF1/CIP1 mRNA in gastric carcinoma and normal mucosa was 93.15% and 76.71% respectively and was signficantly different ( P < 0.05 ). The positive rate of Cyclin D_1 mRNA in gastric carcinoma and normal tissue was 52.05% and 30.16% , and also was significantly different ( P < 0.05 ). The p53 positive rate was 32.87% , the p21 WAF1/CIP1 expression in the p53 positive group was lower than that of the p53 negative group ( P < 0.05 ). The p21 WAF1/CIP1 expression was related to the Cyclin D_1 expression ( P < 0.05 ). Conclusion p21 WAF1/CIP1 、Cyclin D_1 p53 abnormal expression and the probable interaction within them may have much significance in the carcinogenesis and development of gastric carcinoma.