转化生长因子β_1与乙型肝炎患者肝细胞损伤和肝纤维化的关系

Relationship between Serum TGF-β_1 Level, and Liver Function or Liver Fibrosis in Patients with Hepatitis B

目的 观察慢性乙型肝炎、重型乙型肝炎和肝硬化患者血清转化生长因子β1 (TGF-β1 )与肝细胞损伤、肝纤维化的关系。 方法 采用酶联免疫吸附试验测定 2 0例健康献血员、70例不同临床分型乙肝患者血清 TGF-β1 ,放射免疫法检测血清透明质酸 (HA)、 型前胶原蛋白 (P P)、层粘连蛋白 (L N)及 型胶原 (C )。 结果 (1)慢性乙肝 (轻、中、重度 )、重型乙肝、肝硬化患者血清 TGF-β1 水平 (pg/ m L )分别为 10 9.2 9± 5 5 .5 9,2 10 .0 0±12 0 .74 ,4 97.73± 2 76 .73,1138.4 4± 5 80 .4 5 ,10 17.0 6± 5 0 2 .5 0 (P<0 .0 1,P<0 .0 5 ) ;明显高于对照组 (4 3.0 0±10 .5 1,P<0 .0 1)。 (2 )血清 TGF- β1 升高与血清丙氨酸氨基转移酶 (AL T)、天冬氨酸氨基转移酶 (AST)、凝血酶原活动度 (PTA)、胆碱酯酶 (CHE)相关 (P<0 .0 1,P<0 .0 5 )。 (3)血清 TGF-β1 与 P P、C 、L N、HA呈正相关 (P<0 .0 1,P<0 .0 5 )。 结论 TGF- β1 在肝纤维化的发病机制中发挥重要作用 ,血清 TGF- β1

Objective\ To observe the relationship between serum transforming growth factor\|β\-1(TGF\|β\-1) level and liver function or liver fibrosis in patients with hepatitis B.\ Methods\ Serum TGF\|β\-1 was detected by ELISA in 35 patients with chronic hepatitis B(CH), 18 patients with severe hepatitis B(SH) and 17 patients with liver cirrhosis(LC).\ 20 healthy persons served as normal control(NC), meanwhile serum level collagen type Ⅳ(CⅣ), laminin(LN), procollagen peptide(PⅢP), hyaluronic acid(HA) were detected by radioimmunoassay(RIA).\ Results\ (1)Serum TGF\|β\-1 levels in CH, SH and LC were notably higher than that in NC(43 00±10 51 pg/mL, P<0.01) and increased by degrees in mild, moderate, severe of CH, LC and SH(109 29±55 59, 210 00±120 74, 497 73±276 73, 1017 06±502 50, 1138 44±580 45 pg/mL, P<0 01 or P<0 05).\ (2)Serum TGF\|β\-1 was positively correlated with the serum liver function indexes: ALT, AST, CHE, PTA.\ (3)Serum TGF\|β\-1 was positively correlated with serum levels of PCⅣ, LN, PⅢP, HA(P<0.01 or P<0.05).\ Conclusion\ Level of serum TGF\|β\-1 of patients with hepatitis B in CH, SH and LC are consistent with serum indexes of liver fibrosis and liver function