不同条件对大鼠脑胶质瘤C6细胞HSP70表达的诱导

Expression of HSP70 on C6 Globlastoma Cells under Different Conditions

目的 探索和建立 SD大鼠胶质瘤细胞 (C6 ) HSP70蛋白表达最佳诱导条件。 方法 用 MTT法观察不同热休克条件和 /或药物诱导下 C6细胞生存率 ;用 FCM法检测 C6细胞膜 HSP70蛋白的表达。 结果 (1) C6细胞在 4 2 ,4 3℃热休克 0～ 12 h的过程中 ,其生存率无明显影响。各种实验用药物 ,只要加上热休克 4 3℃ 2 h,对C6细胞的生存率的影响较单种药物同浓度要明显。 (2 ) C6细胞经 4 3℃热休克 1,2 ,4 ,6 ,8,10 ,12 ,14 ,16 ,2 0 ,2 4 h后 ,其胞膜 HSP70蛋白表达率均较对照组 (平均 4 .72 % )显著增高 (P<0 .0 5 ) ,其中 12 h表达率 (89.15 % )为最高 ,榄香烯 +热休克组又较其他各组高。 结论 一定条件下的热休克及某些药物可诱导 C6细胞膜 HSP70蛋白的表达 ,其中单因素以 4 3℃热休克 12 h表达率为最高 ,多因素以榄香烯 +43℃热休克 2

Objective\ To establish the optimum conditions for inducing the expression of membrane protein heat\|shock proteins 70(HSP70) of rat C6 glioblastoma cells.\ Methods\ Flow cytometry was applied to determine the kinetic expression of membrane HSP70 of rat C6 glioblastoma cells under different heat shock conditions and/or the treatment with tumoricidal agents β\|elemene, Kang\|lai\|te injection or mitomycin C.\ The survival of C6 cells was determined by MTT assay.\ Results\ The cell survival rate has no significant change subjected to heat\|shock treatment at 42 or 43 ℃ within 12 hours.\ The expression rate of membrane HSP70 on C6 cells at 43 ℃ for 1, 2, 4, 6, 8, 10, 12, 14, 16, 20, 24 hours was 17 57%, 28 19%, 37 35%, 61 38%, 73 19%, 88 61%, 89 15%, 70 18%, 49 00%, 40 17% and 38 85%, respectively.\ These expression percentages were significantly higher than those detected in control group(4 72% in average, P<0 05).\ The expression rate of membrane HSP70 on C6 cells with β\|elemene combined with heat shock treated at 43 ℃ for 12 hours shows the highest among those with other drugs and/or heat shock.\ Conclusion\ Proper heat shock treatment and/or some drugs can increase the expression of HSP70 on C6 cells.