舟山眼镜蛇毒细胞毒素-F的体内抗肿瘤作用

Antitumor Effects of Naja atra Venom Cytotoxin-F

目的 观察舟山眼镜蛇毒细胞毒素 - F(CTX- F)对小鼠肉瘤 180 (S180 )、宫颈癌 U14、艾氏腹水癌(EAC)和 P388白血病的体内抗肿瘤活性。 方法 CTX- F不同剂量腹腔注射 (ip)对 EAC和 P388白血病荷瘤小鼠存活时间、生命延长率的影响 ;CTX- F不同剂量静脉注射对 U 14和 S180荷瘤小鼠瘤块重量的影响 ,并计算抑瘤率。 结果 CTX- F 0 .6和 0 .8m g/ kg实验第 1、3和 5天给药 (ip) ,使艾氏腹水癌荷瘤小鼠存活时间由 10 .0± 2 .0d分别提高到 2 2 .2± 6 .3和 31.3± 9.2 d,生命延长率分别为 12 5 .5 %和 2 13.2 % ;P388小鼠存活时间从 10 .8± 1.9d分别延长到 15 .9± 1.9和 17.6± 2 .3d,生命延长率分别为 4 7.0 3%和 6 3.2 4 %。CTX- F 0 .8和 1.0 m g/ kg静脉注射 ,连续给药 7d,对宫颈癌 U 14抑瘤率分别为 2 6 .6 4 %和 38.87% ;对肉瘤 S180的抑瘤率分别为 31.8%和39.7%。 结论 舟山眼镜蛇毒 CXT-

Objective\ To investigate the antitumor activity of cytotoxin\|F(CTX\|F) from Naja \{atra\} venom in vivo.\ Methods\ Uterus cervix cancer U14 and Carcinoma S180 bearing mice were treated with CTX\|F intravenously; Ehrilich ascitic carcinoma(EAC) bearing mice and P388 leukemia in DBA/2 mice were treated with CTX\|F intraperitoneally.\ Results\ CTX\|F 0 8 and 1 0 mg/kg iv inhibited the growth of U14 and S180 in mice.\ The rates of inhibition of mice bearing U14 were 26 64% and 38 87%, mice bearing S180 were 31 8% and 39 7% respectively.\ CTX\|F 0 6 and 0 8 mg/kg ip also showed regression of ascitic tumors in mice and significant increased life span and mean survival time in EAC and P388 bearing mice.\ The mean survival time of mice bearing EAC was prolonged from 10 0±2 0 d to 22 2±6 3 and 31 3±9 2 d, and mice bearing P388 was from 10 8±1 9 d to 15 9±1 9 and 17 6±2 3 d respectively.\ Conclusion\ CTX\|F exhibited significant antitumor effects in tumor\|bearing mice.