电针对慢性应激抑郁模型大鼠大脑皮层5-HT_1和5-HT_2受体数量和结合活性的影响

Effects of electroacupuncture on receptor number and binding activity of 5-HT _1 and 5-HT_2 in the cerebral cortex of the chronic stress depression modelr ats

目的 :探讨电针“百会”“足三里”穴对抑郁症的调节作用。方法 :复制慢性应激抑郁大鼠模型 ,施以电针“百会”“足三里”穴治疗。采用美国GraphPadPrism 3 0 2专用统计软件 ,以非线性回归方法计算最大结合率 (Bmax)和平衡解离常数 (Kd) ,推测 5 HT1A和 5 HT2A的受体数量与结合活性。结果 :(1 )慢性应激抑郁模型大鼠大脑皮层 5 HT1受体的Bmax值明显低于正常组 (P <0 0 1 ) ,电针可增加其Bmax值。 (2 )抑郁模型大鼠大脑皮层 5 HT2 受体的Bmax值明显高于正常组 (P <0 0 1 ) ,电针可降低其Bmax值 ;抑郁模型大鼠 5 HT2 受体的Kd也高于正常组 (P <0 0 5 ) ,电针治疗后Kd值也有所下降。结论 :5 HT受体功能不平衡是导致抑郁症的重要原因之一 ,电针“百会”“足三里”

Objective To observe the regulatory action of electroacu puncture on depression. Methods Chronic stress depression rat model was established and then was treated by electroacupuncture at Baihui(GV 20) and Zusanli (ST 36). 3H-5-HT or 3H-spiperon were used as specific lig ands for 5-HT 1A or 5-HT 2A receptor respectively to form a saturati on curve, by which percentage of maximal binding (Bmax) and balance separation c onstant (Kd) of receptor -ligand could be calculated with statistical software (GraphPad Prism 3 02), conjecturing number and binding activity of 5-HT 1A and 5-HT 2A receptors. Results (1) Bmax of 5-HT 1 in the s tress model rat was markedly decreased than the normal rat (P<0 01),which c ould be increased by electroacupuncture; (2) Bmax and Kd of 5-HT 2 in the stre ss model rat was markedly increased than the normal rat (P<0 01), which could be decreased by electroacupuncture. Conclusion The imbalance of function of 5-HT receptors might be an important reason for depression, in which electroacu puncture can play a regulatory action.