高效液相色谱法测定消银片含量

Determination of Assay of Xiaoyin Tablet by HPLC With UV Detection

目的:用高效液相色谱法测定消银片含量;方法:ODS 柱,以乙腈:00.1%磷酸溶液(40:60)为流动相,于220nm 波长处检测,流速1.0ml/min;结果:该方法在50μg/ml～460μg/ml 范围内,峰面积与浓度呈良好的线性关系(r=0.9998),重复性试验 RSD=1.21%(n=6);结论:方法简便快速、重现性好。

Objective.Detemination assay of Xiaoyin Tablet by HPLC.Method:Using an ODS column(4.6×250mm).the mobile phase of acetonitrile-0. 3%phosphoric was pumped at 1.0ml/ min.through the column,the detector was 215nm.Standard curve of peak area and concentration was linear in the concentration range of 50μg/ml～460μg/ml-simple,rapid,sensitive and repro- ducible.