豫医无毛小鼠中期染色体标本中无毛基因的原位PCR检测

Detection of Yuyi Hairless Mice’s Hairless Gene in the Slides of Metaphase Chromosome by PCR in Situ

目的 确立豫医无毛小鼠无毛基因的原位PCR荧光检测方法。方法 应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测 ,并设立PCR反应液中无TaqDNA聚合酶、无引物、无bio - 11-dUTP等进行多组对照。结果 染色体标本上出现 1～ 2个黄绿色的扩增信号 ,而对照组则无。

Objective To establish the method of PCR in situ for detection of Yuyi hairless mice's hairless gene. Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.Results Positive signals were detected in the slides of metaphase chromosome of Yuyi hairless mice, but not in control groups. Conclusions The result paves the way for accurate mapping of hairless gene of YYHL.