含神经生长因子的壳聚糖导管桥接周围神经缺损的实验研究

The experimental study of bridging of peripheral nerve defect by chitosan-NGF nerve guide tube

目的 应用含有神经生长因子 (NGF)的壳聚糖神经导引管作为神经再生室桥接大鼠坐骨神经缺损 ,观察其对神经再生的作用。方法 选用Wistar大鼠 6 0只 ,手术造成右后肢坐骨神经长约15mm的缺损 ,A组以含有NGF的壳聚糖神经导引管桥接神经缺损 ,B组则单纯采用壳聚糖导管 ,分别于术后 4、12、2 4周进行大体及显微解剖观察、组织学检查、电镜观察和神经电生理测定。结果 A组在促进神经再生、加快血管化进程、再生神经纤维排列规律化、提高再生神经髓鞘化、加速再生神经功能重建等方面均优于B组。结论 壳聚糖神经导引管可以为大鼠坐骨神经再生提供一个良好的再生微环境 。

Objective To observe the nerve regeneration by using chitosan nerve growth factor(NGF) nerve guide as tubing chambers to bridge the sciatic nerve defects of rats.Methods 60 Wistar rats were chosen randomly , A 15mm defect was produced in the right sciatic nerve of each rat. Chitosan NGF(group A) and chitosan (group B) nerve guides were used to bridge the nerve defects. Gross observation, microanatomical observation , histological examination, electro microscopic examination and nerve electrophysiological examination were carried out at 4, 12, 24 week postoperatively. Results The results of nerve regeneration, vascularization , nerve fibers arrangement, myelination, nerve function reconstruction in group A were better than those in group B.Conclusion The regenerated sciatic nerves of rats are provided with good regeneration microcircumstance by chitosan nerve guide, and NGF can accelerate the regeneration.